PDF Publication Title:
Text from PDF Page: 040
Copyright © Tarek Kakhia. All rights reserved. http://tarek.kakhia.org 5 . 3 . Inhibition of DNA replication Recently it has been proposed that bacterial inactivation by HOCl is the result of inhibition of DNA replication. When bacteria are exposed to HOCl, there is a precipitous decline in DNA synthesis that precedes inhibition of protein synthesis, and closely parallels loss of viability . During bacterial genome replication, the origin of replication ( oriC in E. Coli ) binds to proteins that are associated with the cell membrane, and it was observed that H OCl treatment decreases the affinity of extracted membranes for oriC , and this decreased affinity also parallels loss of viability. A study by Rosen et al.[40] compared the rate of H OCl inhibition of DNA replication of plasmids with different replication origins and found that certain plasmids exhibited a delay in the inhibition of replication when compared to plasmids containing oriC. Rosen’s group proposed that inactivation of membrane proteins involved in DNA replication are the mechanism of action of H OCl . 5 . 4 . Protein Unfolding and Aggregation H OCl is known to cause post-translational modifications to proteins, notably cysteine and methionine oxidation. A recent examination of H OCl's bactericidal role revealed it to be a potent inducer of protein aggregation. Hsp 33 , a chaperone known to be activated by oxidative heat stress, protects bacteria from the effects of H OCl by acting as a holdase , effectively preventing protein aggregation. Strains of E. coli and Vibrio cholerae lacking Hsp 33 were rendered especially sensitive to H OCl . Hsp 33 protected many essential proteins from aggregation and inactivation due to H OCl, which is a probable mediator of H OCl's bactericidal effects . 6 . Safety HOCl is a strong oxidizer and can form explosive mixtures. 41PDF Image | Chlorine Characteristics
PDF Search Title:
Chlorine CharacteristicsOriginal File Name Searched:
Chlorine_17.Tarek_Kakhia.pdfDIY PDF Search: Google It | Yahoo | Bing
Salgenx Redox Flow Battery Technology: Power up your energy storage game with Salgenx Salt Water Battery. With its advanced technology, the flow battery provides reliable, scalable, and sustainable energy storage for utility-scale projects. Upgrade to a Salgenx flow battery today and take control of your energy future.
CONTACT TEL: 608-238-6001 Email: greg@salgenx.com (Standard Web Page)