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International Conference on Chemical Innovation (ICCI 2021) IOP Publishing Journal of Physics: Conference Series 2266 (2022) 012004 doi:10.1088/1742-6596/2266/1/012004 physical properties and chemical composition of hypochlorous acid produced vary depending on the content of NaCl solution, amperage level, time of electrolysis, temperature, and pH [7,8]. EW has received much interest as a chlorine-free alternative to other disinfectants and sanitizers. Acidic EW has received the most attention because of its highly effective antibacterial activity as a main agent, which may penetrate cells and oxidize important metabolic components [9]. EW is identified as the best way to save money on chemicals and is considered environmentally friendly because it simply uses tap water and NaCl [10]. Furthermore, treating or disposing of the chemicals will only have a negative impact on the environment and increase operating costs. Because EW may be generated on- site and does not require any storage space, it is seen as a potential solution to the storage problem [11]. Moreover, a century has passed since the first discovery of EW [5]. EW was first used in agriculture in the 1950s. The use of EW in drinking water disinfection and wastewater treatment plants was proposed in the late 1970s and early 1980s [1]. EW was also developed in Russia, where it was utilized in medical institutes for water decontamination, water regeneration, and disinfection [2]. After that, in the 1980s, EW was commercially introduced in Japan's food sector as sanitation water and an automatic dispenser in the food processing and soda industries. Since then, EW has caught the interest of many researchers, and various publications on its effectiveness as a disinfectant in the food sector have been discovered [12]. Concerns regarding foodborne pathogens identified on raw vegetables in the late 1990s prompted researchers to investigate and explore alternate sanitizers such as EW. With recent developments in technology and the availability of better equipment, EW has benefited from the electrolysis of NaCl aqueous solution. HOCl production and application have been the focus of many researchers. HOCl has been utilized in the electrolysis process to generate chlorine-based antiseptics by mixing drinking water with small amounts of sodium hydroxide at various concentrations. According to the findings, 0.005-0.01 percent HOCl water may destroy most bacteria and pathogens in 12 seconds [13]. The ability of neutral EW as a sanitizer to reduce biofilms of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes on the surface of tomato, plastic, and wooden cutting boards has been demonstrated [14,15]. Red radish seedlings infected with Listeria monocytogenes were treated with EW at a dosage of 60 to 80 ppm, and the germination rate increased from 93.5 percent to 97.7% [16]. After soaking fruit in an EW water solution for as little as 10 minutes, fruit rot caused by the fungus Botryosphaeria berengeriana was suppressed in pears [17]. EW contains antifungal properties and can lower Candida albicans biofilm levels on denture resins: a denture storage and disinfection device [18]. EW has lately been recommended as the disinfectant of choice in an oral and maxillofacial surgery office [19] based on a recent study. The effect of various electrolyzing factors on the formation of HOCl has been described in the literature [20], including electrode types, electrode conductivity, NaCl concentration, salt type, flowrate, temperature, electrical potentials, and electrolysis time. Due to its corrosion-resistant properties of stainless steel, brass, and aluminium as anodes in water electrolysis, many studies on electrode type have been conducted. Unfortunately, when stainless steel corrodes, hazardous chemicals are released, brass deposits copper on the cathode, speeding up the rusting of steel, and aluminium corrodes quickly. Because of their inexpensive cost, higher electrical and thermal conductivity, and relative inertness in alkaline solution compared to metals, carbon-based electrodes such as carbon and graphite are far more practical and attractive [21]. The effect of NaCl was described as follows: lower NaCl concentration reduces HOCl production, which is directly proportional to antibacterial efficacy and inversely proportional to corrosion and biologic compatibility [4]. By electrolysis of NaCl aqueous solution in a single cell electrolyzer, this research project aims to produce a safe disinfectant and sanitizer HOCl. This study project's scope was divided into two parts. The first part focuses on laboratory work that examined at significant factors that affect the electrolysis of NaCl aqueous solution to produce HOCl. Using a UV-vis spectrophotometer, the second part focused on HOCl testing and analysis to determine the effectiveness in killing bacteria. 3PDF Image | Electrolysis Study Effect on Electrolyzed Water
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