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International Conference on Chemical Innovation (ICCI 2021) IOP Publishing Journal of Physics: Conference Series 2266 (2022) 012004 doi:10.1088/1742-6596/2266/1/012004 3.3 The effect of HOCl in reducing bacteria growth In the turbidimetric method, a dense population of bacteria in a sample cuvette will reflect the light that passes through the sample cuvette in a UV-vis spectrophotometer. In other words, the intensity of the original light passing through the sample cuvette will reduce proportionally with the density of bacteria. Thus, if the transmittance is 1, which means the intensity of incoming light is equal to the intensity of light after passing through the sample cuvette, it is an indication of no bacteria growth. If the value of transmittance is near to zero, it means that the bacteria count in the sample is very dense. Table 2 illustrates the transmittance of 6 samples containing 5 mL bacteria solution with bacteria food and 500 ppm HOCl in a varying volume of 0, 2.5, 5, 7.5, 10.0, and 12.5 mL HOCl solution in each sample. All of the samples were incubated at 35°C for 24 hours to allow bacteria to grow before being tested for transmittance in a UV-vis spectrophotometer. Table 2. Uv-vis spectrophotometer transmittance value based on HOCl volume Volume HOCl (ml) 0 2.5 5 7.5 10 12.5 Transmittance 0.194 0.363 0.407 0.651 0.932 1 The transmittance measured after 24 hours of incubation when no HOCl solution was added was 0.194, indicating that the bacteria population was growing. The sample was cloudy due to the presence of a high bacteria population, as detected by thorough observation. The following sample consisted of 2.5 mL of HOCl solution. The measured transmittance was increased slightly to 0.363. In comparison to the first sample, this value indicates that the bacteria population was less dense. According to Table 2, the measured transmittance trend increased as the amount of HOCl solution increased from 0 to 12.5 ml, indicating that the population of bacteria decreased as the HOCl volume was raised. The transmittance was 1.0 at 12.5 ml HOCl, indicating that there were no microorganisms in the sample. This simple but successful procedure demonstrated that HOCl can inhibit bacterial growth. 4. Conclusions The effects of the amount of NaCl and the type of electrodes on the electrolysis of NaCl aqueous solution to produce HOCl were studied in this research project. The results show that 10 grams of NaCl produces 50-200 ppm of HOCl, which is good for sanitizing, and 30 grams of NaCl produces 500-800 ppm of HOCl, which is good for disinfecting. Graphite was chosen as the best electrode because it has been shown to create clear HOCl solutions. The HOCl generated was proven to kill or reduce bacteria growth by using a simple transmittance method. Acknowledgement The researcher grateful and would like to express sincerely to University College TATI for supporting this research project. References [1] Farah R I and Ali S N A 2021 "Electrolyzed Water Generated On-Site as a Promising Disinfectant in the Dental Office During Covid-19" Pandemic Frontiers in Public Health 9:629142 [2] Hricova D, Stephan R and Zweifel C 2008 "Electrolyzed water and its application in the food industry" Journal of Food Protection 71:9 pp. 1934–1947 [3] Huang Y, Hung Y, Hsu S, Huang Y and Hwang D 2008 "Application of electrolyzed water in the food industry" Food Control 19 pp. 329-345 7PDF Image | Electrolysis Study Effect on Electrolyzed Water
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