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Traditionally, there have been several strategies for preventing or removing biofilm, including: Using bactericidal compounds to chemically kill bacteria Using liquid or gas dispersants to break up the biofilm Using mechanical means to physically remove biofilm Using enzymes or chelants to weaken the biofilm structure In the past, no traditional strategies have been proven to be completely effective against biofilm. Even high levels of disinfectant cannot eliminate pathogens within even small amounts of scale. Because biofilm is attached to the substrate by virtue of an electrical charge, dislodgement must be accomplished by disturbance of the charge – therefore very few chemicals are effective. Now, researchers have been demonstrating the success of the use of electrochemically-activated water as an effective alternative for removing biofilm. Results of the studies described below demonstrate the efficacy of NEW on the complete removal of biofilm from surfaces and tubing. 5.1.1 Study: Biofilm Removal from Surfaces A study was performed to research the removal of mature biofilm by application of electrochemically-activated water. Figure 2a shows the mature biofilm control. Results show that exposure of the biofilm to a 1:00 dilution of electrochemically activated water did not yield any noticeable removal of the biofilm (Fig 2b); however, a 1:10 dilution and an undiluted solution resulted in the dispersion and removal of the biofilm after a 20 min exposure (Figures 2c and 2d). Figure 2a: Mature Biofilm control Figure 2b: 1:100 Dilution Figure 2c: 1:10 Dilution Figure 2d: Undilute (100% anolyte) 11 | P a g ePDF Image | Neutral Electrolyzed Water
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