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5.1.2 Study: Biofilms Removal from Tubing A study was performed to demonstrate the removal of biofilm from 12-year-old tubing from the return line of a boat. The original piece of tubing was cut in half, and each half was placed in either H2O or freshly generated NEW (HOCl). The pieces of tubing were allowed to incubate at room temperature for one hour. Results show complete removal of biofilm for the portion of tubing soaked in NEW. This proves that NEW can safely and effectively be used to remove biofilm from lines in any industrial application, instead of using dangerous chemicals, which may leave behind toxic residuals. Figure 3a: Untreated Tubing Figure 3b: Severed Untreated Tubing Figure 3d: Removal of Biofilm by NEW Figure 3c: Incubation in H2O or NEW (HOCl) 5.2 Microorganisms NEW has a strong bactericidal effect on most known pathogenic bacteria due to its high oxidation-reduction potential (ORP > 800 mV). This non-toxic oxidized antimicrobial solution capable of killing many pathogens in less than a minute. Since NEW consists of both HOCl and OCl-, it is believed that the bactericidal action exhibited is due to the combination of these substances. Based on various bactericidal, fungicidal and Sporicidal testing performed to date; NEW has proven to be a high-level disinfectant, substantially reducing pathogens without the use of costly toxic chemicals. Destruction of most vegetative cells of bacteria, molds, and parasites is usually not difficult because they are sensitive to heat and chemicals. However, many organisms excrete extra cellular polysaccharides and form biofilm that protect individual cells, and can be quite difficult to remove from surfaces and manufacturing equipment using traditional methods and chemicals. Studies have shown that NEW can remove biofilm and scale from manufacturing equipment, thus greatly minimizing a major contributor to contamination problems. Molds, Clostridium spp., and Bacillus spp. form spores that are resistant to heat, acids, and other compounds. Parasites also produce resistant cells called cysts, and virus particles are notoriously resistant to heat, chemicals, and drying. NEW has been proven to be an effective Sporicidal, and provides rapid reduction of spores and cysts. Over time, microbes evolve to become resistant to some sanitizers and disinfectants. Because NEW effectively destroys microorganisms, they cannot build up resistance to NEW as they can to other sanitizers and disinfectants. 12 | P a g ePDF Image | Neutral Electrolyzed Water
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